Production of active oxygen and cytotoxicity of tumor cells on pulse laser photodynamic therapy

Author

Nagao, Tomokazu ; Saito, Akira ; Minamitani, Haruyuki ; Iino, Toru ; Yamamoto, Takayuki ; Aizawa, Katsuo

Author_Institution

Fac. of Sci. & Technol., Keio Univ., Yokohama, Japan

Volume

5

fYear

1997

fDate

1997

Firstpage

2296

Abstract

In this study, a doubled Q-switched Nd:YAG laser which has high peak power and lower thermal effect than that of a continuous wave laser, was employed to investigate the damage of tumor cells. As a photosensitizer, zinc coproporphyrin III (Zn CP-III) was prepared. Active oxygen from the photosensitizer was detected by method of luminol chemiluminescence at 430 nm-wavelength. Viability of human small cell carcinoma (MS-1) against the irradiation time, the concentration of Zn CP-III and the pulse energy was investigated. As the result, Zn CP-III produced active oxygen and cells treated by PDT showed a significant reduction of viability

Keywords

cellular effects of radiation; laser applications in medicine; oxygen; photodynamic therapy; tumours; 430 nm; O; YAG:Nd; YAl5O12:Nd; Zn; active oxygen production; cellular radiation biology; continuous wave laser; doubled Q-switched Nd:YAG laser; human small cell carcinoma; irradiation time; luminol chemiluminescence; peak power; photosensitizer; pulse energy; pulse laser photodynamic therapy; thermal effect; tumor cells cytotoxicity; tumor cells damage; viability reduction; zinc coproporphyrin III; Laser excitation; Laser theory; Medical treatment; Neoplasms; Optical pulses; Oxygen; Power lasers; Production; Tumors; Zinc;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1997. Proceedings of the 19th Annual International Conference of the IEEE

Conference_Location

Chicago, IL

ISSN

1094-687X

Print_ISBN

0-7803-4262-3

Type

conf

DOI

10.1109/IEMBS.1997.758822

Filename

758822