Title :
Microfabricated templates for the electrodeposition of metallic barcodes for use in multiplexed bioassays
Author :
True, Randall J. ; Taylor, Micah K. ; Chakarova, Gabriela S. ; Walton, Ian D.
Author_Institution :
Nanoplex Technol., Inc., Menlo Park, CA, USA
Abstract :
Lithographically defined polyimide templates were prepared using microfabrication techniques. The templates consist of high aspect ratio pores that were formed by deep plasma etching. Different metals are electrodeposited into the pores, and then the template is removed to allow the metallic rods (particles) to be released into solution. The striping pattern of metals on the particles is used to optically identify the particles. Particles range in size from 0.6 μm in diameter by 7.7 μm in length to 4.2 μm by 27 μm in length. These particles have been evaluated in a multiplexed DNA hybridization assay.
Keywords :
DNA; biological techniques; electrodeposition; lithography; nanoparticles; nanowires; sputter etching; 0.6 to 4.2 micron; 27 micron; 7.7 micron; DNA hybridization assay; deep plasma etching; electrodeposition; high aspect ratio pores; lithographic; metallic barcodes; microfabricated templates; microfabrication technique; microparticles; multiplexed bioassay; nanoparticles; nanowires; polyimide templates; Bioinformatics; DNA; Etching; Genomics; Nanobioscience; Nanoparticles; Niobium compounds; Plasma applications; Polyimides; Probes; DNA hybridization; ECR plasma etching; bioassays; electrodeposition; high aspect ratio; microparticles; multiplexing; nanoparticles; nanowires; polyimide;
Conference_Titel :
Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE
Conference_Location :
San Francisco, CA
Print_ISBN :
0-7803-8439-3
DOI :
10.1109/IEMBS.2004.1403752
