A rapid, whole blood immunoassay using metal nanoshells

Author

Hirsch, L.R. ; West, J.L. ; Jackson, J.B. ; Lee, A. ; Halas, N.J.

Author_Institution

Dept. of Bioeng., Rice Univ., Houston, TX, USA

Volume

4

fYear

2003

fDate

17-21 Sept. 2003

Firstpage

3442

Abstract

Using metal nanoshells as an immunoassay substrate, we describe an immunoassay capable of detecting blood borne analyte on the order of minutes. Near infrared resonant gold nanoshells were labeled with antibodies specific to rabbit IgG analyte. The antibody-nanoshell conjugates were detectable via near infrared photometry in solution with saline, serum, and whole blood. Addition of analyte induced aggregation of antibody-nanoshell conjugates, causing a decrease in the original nanoshell near infrared resonance. Aggregation proceeded in a concentration dependent fasion in all three mediums (saline, serum, whole blood), permitting quantitative detection of analyte within 10-30 minutes and sensitivities <1 ng/mL.

Keywords

biological techniques; blood; chemical analysis; coagulation; drug delivery systems; gold; nanotechnology; photometry; proteins; substrates; 10 to 30 min; Au; aggregation; antibodies; antibody-nanoshell conjugate; blood borne analyte; blood immunoassay; metal nanoshells; near infrared resonant gold nanoshells; photometry; quantitative detection; rabbit IgG analyte; saline; serum; substrate; Biomedical optical imaging; Blood; Gold; Immune system; Nanoparticles; Optical attenuators; Optical sensors; Optical variables control; Rabbits; Resonance;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2003. Proceedings of the 25th Annual International Conference of the IEEE

ISSN

1094-687X

Print_ISBN

0-7803-7789-3

Type

conf

DOI

10.1109/IEMBS.2003.1280886

Filename

1280886