Workshop: Transcriptome assembly from RNA-Seq data: Objectives, algorithms and challenges

In this talk, IsoLasso , a new RNA-Seq based transcriptome assembly tool is introduced. IsoLasso is based on the well-known LASSO algorithm, which is a multivariate regression method designated to seek a balance between the maximization of prediction accuracy and the minimization of interpretation. By including some additional constraints in the quadratic programming involved in LASSO, IsoLasso is able to make the set of assembled transcripts as complete as possible. Experiments on simulated and real RNA Seq datasets show that IsoLasso achieves higher sensitivity and precision simultaneously than other recently released transcriptome assembly algorithms including Isolnfer , Cufflinks and Scripture.