Title :
A highly-simplified nucleic-acid microfluidic test device based on capillary wicking action for point-of-care diagnostics
Author :
Varapula, D. ; Gutta, S. ; Mauk, M.G. ; Liu, C.
Author_Institution :
Drexel Univ., Philadelphia, PA, USA
Abstract :
On-chip devices for point-of-care diagnostics are excellent options for rapid and low-cost testing. However, there are not many fully integrated on-chip nucleic-acid tests reported in literature. Here, we propose a palm-sized `chip´ in which solid-phase extraction, isothermal enzymatic amplification (LAMP) and real-time fluorescence detection are integrated. The device operates with one moving part; fluid actuation and control is effected by capillary wicking action of absorbent materials, requiring no external pumping or power.
Keywords :
bioMEMS; biochemistry; capillarity; enzymes; fluorescence; lab-on-a-chip; microfluidics; molecular biophysics; patient care; patient diagnosis; real-time systems; LAMP; absorbent material; capillary wicking action; external power; external pumping; fluid actuation; fluid control; fully integrated on-chip nucleic-acid test; highly-simplified nucleic-acid microfluidic test device; isothermal enzymatic amplification; on-chip device; palm-sized chip; point-of-care diagnostics; rapid low-cost testing; real-time fluorescence detection; solid-phase extraction; Biomembranes; DNA; Fluids; Fluorescence; Protocols; System-on-chip; Testing;
Conference_Titel :
Biomedical Engineering Conference (NEBEC), 2015 41st Annual Northeast
Conference_Location :
Troy, NY
Print_ISBN :
978-1-4799-8358-2
DOI :
10.1109/NEBEC.2015.7117131