Title :
Analysis of elasticity and deformability of erythrocytes using micro-channel flow system and atomic force microscope
Author :
Minamitani, Haruyuki ; Tsukada, Kosuke ; Kawamura, Tomoni ; Sekizuka, Eiichi ; Oshio, Chikara
Author_Institution :
Fac. of Sci. & Technol., Keio Univ., Yokohama, Japan
Abstract :
The mechanical properties of erythrocytes under diabetic and some other physico-chemical conditions were measured by using a transparent micro-channel flow system for the analysis of erythrocyte deformability and also by using atomic force microscope (AFM) for the analysis of microscopic elasticity of the erythrocyte cell membrane respectively. The deformability of diabetic erythrocytes was significantly lower than that of healthy controls, especially at higher shear rates. The spring constant and the Young´s modulus of diabetic erythrocytes were much higher than those of healthy controls. These erythrocytes were obviously stiffer than normal and they would be hard to deform in the capillary flow passage. This may lead to the growth of microangiopathy
Keywords :
Young´s modulus; atomic force microscopy; biomedical measurement; cellular biophysics; diseases; elasticity; haemorheology; mechanical variables measurement; microfluidics; capillary flow passage; cell mechanical properties; diabetic conditions; erythrocytes deformabilty; erythrocytes elasticity; healthy controls; microangiopathy growth; physicochemical conditions; red blood cells; spring constant; transparent microchannel flow system; Atomic force microscopy; Atomic measurements; Biomembranes; Cells (biology); Diabetes; Elasticity; Fluid flow measurement; Force measurement; Mechanical factors; Mechanical variables measurement;
Conference_Titel :
Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000
Conference_Location :
Lyon
Print_ISBN :
0-7803-6603-4
DOI :
10.1109/MMB.2000.893743
