Development of microfluidic channels for the culture of mammalian embryos

Author

Davis, J.A. ; Raty, S. ; Eddington, D.T. ; Glasgow, I.K. ; Zeringue, H.C. ; Wheeler, M.B. ; Beebe, D.J.

Author_Institution

Dept. of Electr. & Comput. Eng., Illinois Univ., Urbana, IL, USA

fYear

2000

fDate

2000

Firstpage

307

Lastpage

310

Abstract

Microfluidic channels are explored as a tool for the culture of mammalian embryos. Three different materials are used to construct microfluidic devices for embryo culture: silicon, glass, and polydimethylsiloxane. Practical issues including, loading, localization-visualization and retrieval of the embryos in the microchannels, bubble formation within the device, and development of cleaning and sterilization procedures are discussed and addressed via design iterations. Preliminary data on embryo development within the microchannels have been collected

Keywords

biological techniques; bubbles; glass; microfluidics; silicon; zoology; cleaning procedures; design iterations; embryo culture; embryos retrieval; loading; localization; mammalian embryos culture; microfluidic channels development; microfluidic devices; polydimethylsiloxane; sterilization procedures; visualization; Animals; Biomedical engineering; Drives; Embryo; In vitro fertilization; In vivo; Laboratories; Microchannel; Microfluidics; USA Councils;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000

Conference_Location

Lyon

Print_ISBN

0-7803-6603-4

Type

conf

DOI

10.1109/MMB.2000.893793

Filename

893793