DocumentCode :
2756193
Title :
DNA sensor. II. Biolayer generation and characterisation
Author :
West, Jonathan ; Laureyn, Wim ; Haefele, Eberhard ; Berney, Helen ; Lane, William
Author_Institution :
Nat. Microelectron. Res. Centre, Cork, Ireland
fYear :
2000
fDate :
2000
Firstpage :
402
Lastpage :
406
Abstract :
For pt. I see ibid., p. 400-1 (2000). A silicon-based sensor for the direct detection of DNA sequences has been developed. Hybridisation of analyte DNA with complementary sequences immobilised on a silicon transducer induces charge effects that alter the dielectric properties of the surface. This phenomena can be detected by the associated change in the measured capacitance. The biosensor demonstrates sequence specificity and a quantitative response, but is presently hindered by limited sensitivity and poor reproducibility. To address these issues, alternative methods for silicon reconfiguration (see pt. I) and biolayer generation were explored. Biolayer fabrication involved generation of a Si-SiO2-Si3N4 surface rich in hydroxyl groups by plasma etching or chemical treatment. Hydroxyl groups were employed for silanisation with 3-aminopropyltriethoxysilane (3-APTES). Three chemistries were used to covalently attach single stranded probe DNA via the 3-APTES terminal amino group and hybridisation ability was evaluated using a fluorescent technique. Once optimised, this sensor can be elaborated into an array of sensors for sample interrogation with multiple probes. Such an array will be used to determine the antimicrobial susceptibility profile of clinical isolates of Mycobacterium tuberculosis
Keywords :
DNA; biosensors; dielectric properties; oxygen compounds; silicon; silicon compounds; sputter etching; 3-APTES terminal amino group; DNA sequences detection; Mycobacterium tuberculosis; Si-SiO2-Si3N4; Si-SiO2-Si3N4 surface rich in hydroxyl groups; analyte DNA hybridisation; antimicrobial susceptibility profile; charge effects; chemical treatment; clinical isolates; immobilised complementary sequences; limited sensitivity; plasma etching; poor reproducibility; quantitative response; sequence specificity; silicon reconfiguration; silicon transducer; single stranded probe DNA; surface dielectric properties alteration; Biosensors; Character generation; Chemical sensors; DNA; Plasma chemistry; Probes; Sensor arrays; Sensor phenomena and characterization; Sequences; Silicon;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000
Conference_Location :
Lyon
Print_ISBN :
0-7803-6603-4
Type :
conf
DOI :
10.1109/MMB.2000.893815
Filename :
893815
Link To Document :
بازگشت