Flow of λ-DNA in microfluidic devices

Author

Shrewsbury, Polly J. ; Muller, Susan J. ; Liepmann, Dorian

Author_Institution

Bioeng. Program, California Univ., Berkeley, CA, USA

fYear

2000

fDate

2000

Firstpage

415

Lastpage

420

Abstract

This research seeks to understand the behavior of biological macromolecules in microfluidic devices. Epifluorescence microscopy was used to observe the conformation of λ-DNA macromolecules during flow through sudden expansions, sudden contractions, straight channels, and a valve. The authors´ analysis indicates that both the flow patterns within the device and the device geometry can influence macromolecular structure and stability. Utilization of this type of analysis in the design of microsystems for biochemical processing is instrumental for maintaining molecular stability and for ensuring accuracy of sample analysis

Keywords

DNA; biological techniques; biorheology; microfluidics; molecular biophysics; λ-DNA flow; DNA conformation; biochemical processing; device geometry; epifluorescence microscopy; flow patterns; macromolecular stability; macromolecular structure; microfluidic devices; microsystems design; molecular stability maintenance; sample analysis accuracy; Biochemical analysis; Biomedical engineering; Biosensors; Chemical and biological sensors; Fabrication; Geometry; Microfluidics; Microscopy; Reservoirs; Valves;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000

Conference_Location

Lyon

Print_ISBN

0-7803-6603-4

Type

conf

DOI

10.1109/MMB.2000.893818

Filename

893818