Title :
On-chip microfluidics for proteomic analysis by electrospray ionization/mass spectrometry
Author :
Tabourier, Pierre ; Druon, Christian ; Rolando, Christian ; Lefebvre, Patricia
Author_Institution :
IEMN, UMR, Villeneuve d´´Ascq, France
Abstract :
Describes the development of a silicon integrated demonstrator coupled to an ElectroSpray Ionization source in view of protein analysis by Mass Spectrometry (ESI/MS). The design and the making of basic components: (i) a novel thermal valveless micropump, (ii) a Solid Phase Extraction (SPE) column and (iii) a hollow cantilever nanoemitter constitute the steps of the authors´ ongoing technological program which will be achieved by their gathering within a single chip. At present a manifold prototype at a 10 μm scale has been fabricated and is currently being tested
Keywords :
biological techniques; mass spectroscopic chemical analysis; microfluidics; molecular biophysics; proteins; 10 mum; Si; electrospray ionization; hollow cantilever nanoemitter; manifold prototype; mass spectrometry; on-chip microfluidics; proteomic analysis; silicon integrated demonstrator; single chip; solid phase extraction column; technological program; thermal valveless micropump; Ionization; Mass spectroscopy; Microfluidics; Micropumps; Protein engineering; Proteomics; Prototypes; Silicon; Solids; Testing;
Conference_Titel :
Microtechnologies in Medicine and Biology, 1st Annual International, Conference On. 2000
Conference_Location :
Lyon
Print_ISBN :
0-7803-6603-4
DOI :
10.1109/MMB.2000.893823
