Title :
Open-access phototransistor-based optoelectronic tweezers for long-term single cell heterogeneity study
Author :
Hsu, H.Y. ; Jamshidi, A. ; Shekarchian, S. ; Lam, W. ; Valley, J.K. ; Pei, S.N. ; Wu, M.C.
Author_Institution :
Electr. Eng. & Comput. Sci., Univ. of California, Berkeley, CA, USA
Abstract :
We demonstrate, for the first-time, long-term culturing of single cells under continuous phototransistor-based optoelectronic tweezers (Ph-OET) trapping. A new device configuration, open-access Ph-OET, is presented to integrate the trapping functionality of Ph-OET with culturing capabilities in a Petri dish. Maintenance of human leukemia cell lines (K562, Jurkat) under optical manipulation for up to five cell generation has been achieved.
Keywords :
biomedical electronics; biomedical engineering; blood; cancer; cellular biophysics; phototransistors; radiation pressure; Petri dish; Ph-OET; human leukemia cell lines; optical manipulation; optoelectronic tweezers; phototransistor; single cell heterogeneity; trapping functionality; Arrays; Biomedical optical imaging; Charge carrier processes; Media; Monitoring; Optical imaging; Substrates;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on
Conference_Location :
Cancun
Print_ISBN :
978-1-4244-9632-7
DOI :
10.1109/MEMSYS.2011.5734362
