Open-access phototransistor-based optoelectronic tweezers for long-term single cell heterogeneity study

Author

Hsu, H.Y. ; Jamshidi, A. ; Shekarchian, S. ; Lam, W. ; Valley, J.K. ; Pei, S.N. ; Wu, M.C.

Author_Institution

Electr. Eng. & Comput. Sci., Univ. of California, Berkeley, CA, USA

fYear

2011

fDate

23-27 Jan. 2011

Firstpage

63

Lastpage

66

Abstract

We demonstrate, for the first-time, long-term culturing of single cells under continuous phototransistor-based optoelectronic tweezers (Ph-OET) trapping. A new device configuration, open-access Ph-OET, is presented to integrate the trapping functionality of Ph-OET with culturing capabilities in a Petri dish. Maintenance of human leukemia cell lines (K562, Jurkat) under optical manipulation for up to five cell generation has been achieved.

Keywords

biomedical electronics; biomedical engineering; blood; cancer; cellular biophysics; phototransistors; radiation pressure; Petri dish; Ph-OET; human leukemia cell lines; optical manipulation; optoelectronic tweezers; phototransistor; single cell heterogeneity; trapping functionality; Arrays; Biomedical optical imaging; Charge carrier processes; Media; Monitoring; Optical imaging; Substrates;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on

Conference_Location

Cancun

ISSN

1084-6999

Print_ISBN

978-1-4244-9632-7

Type

conf

DOI

10.1109/MEMSYS.2011.5734362

Filename

5734362