Micromechanical sensors for the measurement of biopolymer degradation

Author

Keller, S.S. ; Gammelgaard, L. ; Jensen, M.P. ; Schmid, S. ; Davis, Z.J. ; Boisen, A.

Author_Institution

Dept. of Micro- & Nanotechnol., Tech. Univ. of Denmark, Lyngby, Denmark

fYear

2011

fDate

23-27 Jan. 2011

Firstpage

457

Lastpage

460

Abstract

We present microcantilever-based sensors for the characterization of biopolymer degradation by enzymes. Thin films of Poly(L-lactide) (PLLA) were spray-coated onto SU-8 cantilevers with well-known material properties and dimensions. The micromechanical sensors were immersed in solutions of proteinase K to investigate enzymatic degradation of PLLA. A decrease of the resonance frequency after immersion indicated degradation of the biopolymer coating and allowed the estimation of the degradation rate at a specific enzyme concentration.

Keywords

Young´s modulus; bioMEMS; biochemistry; biodegradable materials; biological techniques; cantilevers; enzymes; microsensors; molecular biophysics; polymer films; spray coatings; POLY(L-lactide) thin films; SU-8 cantilevers; biopolymer coating; biopolymer degradation; enzymes; immersion; microcantilever-based sensors; micromechanical sensors; proteinase K; resonance frequency; spray coating; Coatings; Degradation; Frequency measurement; Gold; Proteins; Resonant frequency;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on

Conference_Location

Cancun

ISSN

1084-6999

Print_ISBN

978-1-4244-9632-7

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2011.5734460

Filename

5734460