Title :
Physiological effects of focused ultrasound pulses on mammalian CNS tissue in an in-vitro preparation
Author :
Georgides, C.S. ; Rinaldi, P.C. ; Jones, J.P. ; Price, L. ; Reines, F.
fDate :
Oct. 31 1994-Nov. 3 1994
Abstract :
We have investigated the effects of pulsed and highly focused ultrasound on the physiology of 500 micron thick hippocampal slices obtained from 50 male, 120 gram Sprague-Dawley rats. During the study the slices were kept alive in a special in-vitro preparation. We compared the magnitude and latency of the control evoked responses to those of the responses recorded during ultrasound irradiation. Comparisons were made for both the orthodromic and antidromic population responses. For all studies, a highly focused pulsed ultrasonic beam was used with a center frequency of 5 MHz and an intensity (SPTA) of 52 W/cm 2. Significant changes in both the magnitude and the stimulus-response delay were observed. Full recovery was observed when the ultrasound was removed. In addition, temperature effects could be rejected as the sole mechanism producing such changes
Keywords :
acoustic pulses; biological effects of acoustic radiation; cellular effects of radiation; neurophysiology; ultrasonic effects; ultrasonic focusing; 120 g; 5 MHz; 500 micron; Sprague-Dawley rats; antidromic population responses; control evoked responses; focused ultrasound pulses; full recovery; highly focused ultrasound; hippocampal slices; in-vitro preparation; mammalian CNS tissue; orthodromic population responses; physiological effects; physiology; pulsed ultrasound; stimulus-response delay; temperature effects; ultrasound irradiation; Acoustic pulses; Acoustic radiation effects; Biological effects of acoustic radiation; Biological tissues; Nervous system;
Conference_Titel :
Ultrasonics Symposium, 1994. Proceedings., 1994 IEEE
Conference_Location :
Cannes, France
Print_ISBN :
0-7803-2012-3
DOI :
10.1109/ULTSYM.1994.401947
