Enhancement of Microscopic Images Using Modified Self-Adaptive Plateau Histogram Equalization

In this paper, we modified a method known as self-adaptive plateau histogram equalization (SAPHE) to enhance microscopic images acquired using optical microscope. First, our method decides the plateau threshold value, automatically; based on the histogram itself. Then, using this plateau threshold, the bins of the histogram are modified. Finally, histogram equalization based on this modified histogram is carried out. Experimental results show that this modified SAPHE method is able to enhance the microscopic images without over amplifying the noise level, as compared with global histogram equalization.