Electrodeformation for single cell mechanical characterization

Author

Chen, Jian ; Abdelgawad, Mohamed ; Yu, Liming ; Shakiba, Nika ; Chien, Wei-Yin ; Lu, Zhe ; Geddie, William B. ; Jewett, Michael A S ; Sun, Yu

Author_Institution

Inst. of Biomater. & Biomed. Eng., Univ. of Toronto, Toronto, ON, Canada

fYear

2011

fDate

23-27 Jan. 2011

Firstpage

1119

Lastpage

1122

Abstract

This paper presents the use of electrodeformation as a method for single cell mechanical characterization. Cells were placed between two microelectrodes with a rectangular AC electric field applied and cell deformation recorded. Numerical simulations were performed to model cell electrodeformation based on the Maxwell stress tensor formulation in which effects of cell electrical property variations on their electrodeformed behaviors were investigated. By comparing the measured morphological changes with those obtained from numerical simulations, we were able to quantify Young´s modulus of SiHa cells (601±183 Pa) and ME180 cells (1463±649 Pa), which were then verified using conventional micropipette aspiration (SiHa: 400±290 Pa and ME180: 1070±580 Pa).

Keywords

Young´s modulus; bioelectric phenomena; biological effects of fields; biomechanics; biomedical electrodes; cellular biophysics; deformation; microelectrodes; ME180 cells; Maxwell stress tensor formulation; SiHa cells; Young´s modulus; cell deformation; cell electrical property variation; electrodeformation; microelectrodes; micropipette aspiration; morphological changes; numerical simulation; rectangular AC electric field; single cell mechanical characterization; Biomembranes; Conductivity; Electric fields; Electrodynamics; Force; Young´s modulus;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on

Conference_Location

Cancun

ISSN

1084-6999

Print_ISBN

978-1-4244-9632-7

Type

conf

DOI

10.1109/MEMSYS.2011.5734626

Filename

5734626