Multiplexing of highly reproducible, bead-based immunoassays on a centrifugal microfluidic platform

This work demonstrates for the first time integrated and highly multiplexable immunoassays on single beads on a centrifugal microfluidic platform. Sharply peaked, single-occupancy distributions of the monodisperse beads are achieved by a stopped-flow, merely sedimentation-based introduction of the beads to an array of scale-matched geometrical barriers. Compared to statistically aggregated beads, our here presented single-bead scheme offers homogeneous flow conditions throughout the spatially separated trapping sites.