Title :
UV photodiode based portable fiber optic biosensor
Author :
Dhawangale, Arvind ; Bharadwaj, Reshma ; Mukherji, Soumyo ; Kundu, Tapanendu
Author_Institution :
Dept. of Biosci. & Bioeng., Indian Inst. of Technol. Bombay, Mumbai, India
Abstract :
The strong optical absorbance characteristics around 280 nm of several protein chromophores present in biomolecules like Immunoglobulin (IgG) and cell walls of bacteria can be exploited for direct detection of such biological analytes. In this report, we describe the development of a portable fiber optic biosensor, using a UV LED with peak emission at 280 nm and a UV photodiode to measure evanescent wave absorbance changes. A transimpedance amplifier is used as a (photo) current to voltage converter and for amplification of optical signals from the photodiode. The sensor is able to detect 50 μg/ml of Goat anti-Human Immunoglobulin (GaHIgG), used as a model protein and thus, could provide a suitable, inexpensive platform for label-free detection of water borne pathogen like E. coli cells.
Keywords :
biosensors; cellular biophysics; fibre optic sensors; light absorption; light emitting diodes; macromolecules; microorganisms; molecular biophysics; operational amplifiers; optical fibres; optical signal detection; photodiodes; proteins; ultraviolet detectors; E.coli cells; UV LED; UV photodiode; bacteria; biological analytes; biomolecules; cell walls; evanescent wave absorbance change; goat anti-human immunoglobulin; label-free detection; portable fiber optic biosensor; protein chromophores; strong optical absorbance characteristics; transimpedance amplifier; water borne pathogen; Biomedical optical imaging; Optical fiber sensors; Optical fibers; Photodiodes; Probes; 280 nm; Evanescent wave absorbance; Fiber optic biosensor; UV Photodiode;
Conference_Titel :
Systems in Medicine and Biology (ICSMB), 2010 International Conference on
Conference_Location :
Kharagpur
Print_ISBN :
978-1-61284-039-0
DOI :
10.1109/ICSMB.2010.5735399
