Integrated optofluidic strategies for a system level understanding of pathological states during space flights

In this work, we propose a novel configuration for a microfluidic chip which integrates an optofluidic section devoted to the generation and acquisition of the signal used to determine the pathological status of cells. Cell tissues acquired from a patient are injected in this device and kept fed with a specific medium. At this point tissues are stimulated using 4 different wavelengths to solicit fluorescent proteins to reveal the amount marker disease-associated gene expression. A number of microfluidic channels constitute laser cavities obtained by modifying the dye concentration and using a single pump laser, the optofluidic section of the device. In the proposed configuration a power meter is thought to track the evolution of the fluorescence signal emitted by the cells in the probed tissues so as to allow the recording and the following analysis of the signal.