Title :
Semiconductor quantum dots as highly effective biosensing tools
Author :
Buiculescu, R. ; Chaniotakis, N.A.
Author_Institution :
Dept. of Chem., Univ. of Crete, Iraklion, Greece
Abstract :
In this paper, highly luminescent core/shell CdSe/ZnS quantum dots have been used in the construction of two sensing systems for the detection of oligonucleotide hybridization and enzymatic reaction by covalent binding the biomolecules directly to the surface of the quantum dots. It is shown that the oligonucleotide-modified quantum dots have proven to be able to detect large sequences of mismatched bases through the hybridization process, while the system based on biosilica encapsulated enzyme-modified quantum dots was found to be suitable for monitoring low substrate concentrations in solution and with a storage lifetime of more than 2 months.
Keywords :
II-VI semiconductors; biochemistry; biosensors; cadmium compounds; chemical sensors; enzymes; fluorescence; molecular biophysics; molecular configurations; semiconductor quantum dots; silicon compounds; wide band gap semiconductors; zinc compounds; CdSe-ZnS-SiO2; biomolecules; biosilica encapsulated enzyme-modified quantum dots; covalent binding; enzymatic reaction; highly effective biosensing tools; highly luminescent core-shell quantum dots; low-substrate concentration monitoring; mismatched base sequences; oligonucleotide hybridization detection; oligonucleotide-modified quantum dots; semiconductor quantum dots; storage lifetime; Biochemistry; Fluorescence; Nanocomposites; Phase locked loops; Quantum dots; Silicon compounds; Substrates;
Conference_Titel :
Semiconductor Conference (CAS), 2012 International
Conference_Location :
Sinaia
Print_ISBN :
978-1-4673-0737-6
DOI :
10.1109/SMICND.2012.6400690
