Two-dimensional magnetic droplet manipulation platform for miniaturized bioanalytical applications

Author

Lehmann, U. ; Vandevyver, C. ; Parashar, V.K. ; DeCourten, D. ; Gijs, M.A.M.

Author_Institution

Inst. of Microelectron. & Microsystems, Lausanne

fYear

2007

fDate

21-25 Jan. 2007

Firstpage

521

Lastpage

524

Abstract

We present a new approach for the manipulation of small liquid samples for the miniaturization of bioanalytical systems. In our system, minute liquid volumes are manipulated via the interaction of a changeable magnetic field and magnetic microparticles contained within aqueous droplets. Our setup enables the easy conversion of macroscopic bioanalytical protocols into the lab-on-a-chip format. Here we present the implementation of a DNA extraction and purification protocol as well as the on-chip optical detection of bioactive molecules. In both cases the miniaturization results in a decrease of the reaction and detection time as well as an increase in sensitivity.

Keywords

DNA; biological techniques; biosensors; lab-on-a-chip; magnetic particles; microsensors; molecular biophysics; optical sensors; DNA extraction; DNA purification; aqueous droplets; bioactive molecules; lab-on-a-chip; magnetic field interaction; magnetic microparticles; miniaturized bioanalytical applications; on-chip optical detection; two-dimensional magnetic droplet manipulation; Biomedical optical imaging; DNA; Lab-on-a-chip; Magnetic fields; Magnetic liquids; Micromagnetics; Optical detectors; Optical sensors; Protocols; Purification;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2007. MEMS. IEEE 20th International Conference on

Conference_Location

Hyogo

ISSN

1084-6999

Print_ISBN

978-1-4244-095-5

Electronic_ISBN

1084-6999

Type

conf

DOI

10.1109/MEMSYS.2007.4433119

Filename

4433119