Title :
Development of an Integrated In Situ Analyzer for Quantitative Analysis of Microbial ATP in Aquatic Environments
Author :
Fukuba, T. ; Fukuzawa, N. ; Glutz, L.S. ; Miyaji, A. ; Fujii, T.
Author_Institution :
Univ. of Tokyo, Tokyo
Abstract :
An integrated and miniaturized device for in situ quantitative analysis of microbial ATP was developed in this study. The device can perform extracellular ATP elimination, cell lysis and ATP quantification in a microfluidic chip. A pattern of a microchannel has been optimized to measure luminescence caused by luciferin-luciferase reaction. As a result of evaluation of the developed microfluidic device, 10 nM to 100 muM of ATP was successfully quantified. Environmental samples and bacterial culture media were also analyzed by the microfluidic device. Towards in situ calibration of the ATP quantitative analysis device, a prototype of caged ATP based online calibration component was also fabricated and evaluated.
Keywords :
bioluminescence; luminescence; microfluidics; microorganisms; oceanographic equipment; oceanographic techniques; seawater; ATP quantification; aquatic environment; bacterial culture media; cell lysis; extracellular ATP elimination; integrated in situ analyzer; luciferin-luciferase reaction; luminescence; microbial ATP; microchannel; microfluidic chip; Calibration; Extracellular; Microchannel; Microfluidics; Microorganisms; Pollution measurement; Prototypes; Remotely operated vehicles; Semiconductor device measurement; Temperature;
Conference_Titel :
Underwater Technology and Workshop on Scientific Use of Submarine Cables and Related Technologies, 2007. Symposium on
Conference_Location :
Tokyo
Print_ISBN :
1-4244-1207-2
Electronic_ISBN :
1-4244-1208-0
DOI :
10.1109/UT.2007.370802
