Title :
A method for normalizing histology slides for quantitative analysis
Author :
Macenko, Marc ; Niethammer, Marc ; Marron, J.S. ; Borland, David ; Woosley, John T. ; Guan, Xiaojun ; Schmitt, Charles ; Thomas, Nancy E.
Author_Institution :
Dept. of Comput. Sci., Univ. of North Carolina, Chapel Hill, NC, USA
fDate :
June 28 2009-July 1 2009
Abstract :
Inconsistencies in the preparation of histology slides make it difficult to perform quantitative analysis on their results. In this paper we provide two mechanisms for overcoming many of the known inconsistencies in the staining process, thereby bringing slides that were processed or stored under very different conditions into a common, normalized space to enable improved quantitative analysis.
Keywords :
biological tissues; biology computing; biomedical imaging; 24-bit RGB cameras; histology slide normalization; quantitative analysis; Biological cells; Deconvolution; Detectors; Diffraction; Fluorescence; Image retrieval; Information retrieval; Maximum likelihood estimation; Microscopy; Testing; Biomedical image processing; Biomedical microscopy; Biomedical signal detection; Melanoma; Pathology;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2009. ISBI '09. IEEE International Symposium on
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-3931-7
Electronic_ISBN :
1945-7928
DOI :
10.1109/ISBI.2009.5193250
