Title :
Identification of cellular dynamic patterns resulting from repetitive photobleaching using Independent Component Analysis
Author :
van Gemert, A.M.C. ; Jost, C.R. ; van der Laan, A.M.A. ; Dirks, R.W. ; Reiber, J.H.C. ; Tanke, H.J. ; Lelieveldt, B.P.F.
Author_Institution :
Med. Center, Dept. of Mol. Cell Biol., Leiden Univ., Leiden, Netherlands
fDate :
June 28 2009-July 1 2009
Abstract :
Fluorescence loss in photobleaching (FLIP) experiments are used to determine the dynamic properties of fluorescently labeled macromolecules in cells. This paper presents a novel method to facilitate the interpretation of dynamic patterns in FLIP experiments based on independent component analysis. By decomposing the image sequence into a stationary and a dynamic component, and by deriving a bleaching contrast function, we demonstrate that the triplet of component and bleaching images enhances the interpretation of FLIP sequences by outlining the movement of two fluorescent proteins with well defined dynamic characteristics.
Keywords :
biomedical optical imaging; cellular biophysics; fluorescence; image enhancement; image sequences; independent component analysis; medical image processing; molecular biophysics; optical saturable absorption; proteins; bleaching contrast function; cellular dynamic patterns; fluorescence loss in photobleaching; fluorescent proteins; image sequence; independent component analysis; macromolecules; Biological cells; Biomedical imaging; Bleaching; Fluorescence; Image analysis; Image sequences; Independent component analysis; Medical treatment; Photobleaching; Proteins; Fluorescence Loss In Photobleaching; Independent Component Analysis; ROI definition;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2009. ISBI '09. IEEE International Symposium on
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-3931-7
Electronic_ISBN :
1945-7928
DOI :
10.1109/ISBI.2009.5193323
