Photo-transfection of mammalian cells via femtosecond laser pulses

Author

Mthunzi, Patience ; Gunn-Moore, Frank J. ; Dholakia, Kishan

Author_Institution

Sch. of Phys. & Astron., St Andrews Univ., St. Andrews, UK

fYear

2009

fDate

14-19 June 2009

Firstpage

1

Lastpage

1

Abstract

In this work we report on transient photo-transfection of ovary (CHO-K1), neuroblastoma (NG-108 & SK- N-SH) and embryonic kidney (HEK-293) as well as primary non-differentiated stem cells (E14g2a) using a tightly focused titanium sapphire laser beam (~1.1 mum diameter spot size). Transfection efficiencies between 40 - 63 % are recorded. We show for the first time that, due to their different sensitivity, surface receptors and membrane structure the cell lines mentioned above displayed varying photo-transfection efficiencies at different subculture "passage" numbers.

Keywords

bio-optics; cellular biophysics; laser applications in medicine; embryonic kidney; femtosecond laser pulses; mammalian cells; membrane structure; neuroblastoma; phototransfection; stem cells; surface receptors; Biomedical optical imaging; Biomembranes; Cells (biology); Genetics; Humans; Occupational stress; Optical pulses; Optical sensors; Proteins; Ultrafast optics;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics 2009 and the European Quantum Electronics Conference. CLEO Europe - EQEC 2009. European Conference on

Conference_Location

Munich

Print_ISBN

978-1-4244-4079-5

Electronic_ISBN

978-1-4244-4080-1

Type

conf

DOI

10.1109/CLEOE-EQEC.2009.5194773

Filename

5194773