Effect of fibroblasts proliferation by light-emitting diode stimulation via cytochrome c oxidase of mitochondria

Author

Hsieh, Yue-Feng ; Hsieh, Jui-Hsiang ; Ruaan, Ruoh-Chyu ; Yeong, Eng-kean ; Chou, Yu-Chi

Author_Institution

Dept. of Biomed. Eng., Chung Yuan Christian Univ., Chungli, Taiwan

fYear

2010

fDate

Nov. 30 2010-Dec. 2 2010

Firstpage

134

Lastpage

137

Abstract

The objective of the project was investigated that the relationships between cytochrome c and fibroblasts proliferation. The electron transport from cytochrome a3 to CuB was blocked by sodium azide and the action of Na⁺, K⁺-ATPase in cell membrane was inhibited by ouabain. Light emitting diode was stimulated after sodium azide and ouabain treatment to investigate whether cytochrome c was related to fibroblasts proliferation. We also realized whether two inhibitors affected ATP synthesis at the same time. The results will be realized whether fibroblasts proliferation by light emitting diode stimulation was related to ATP synthesis in mitochondria. Furthermore, the mechanism of fibroblasts proliferation by light emitting diode stimulation was also demonstrated.

Keywords

bio-optics; bioelectric phenomena; biological effects of fields; biomembranes; cellular biophysics; enzymes; light emitting diodes; molecular biophysics; K⁺-ATPase; Na⁺-ATPase; cell membrane; cytochrome C oxidase of mitochondria; electron transport; fibroblast proliferation; light-emitting diode stimulation; mitochondria; ouabain; sodium azide; Chemicals; Fibroblasts; Inhibitors; Laser excitation; Light emitting diodes; Radiation effects; Semiconductor laser arrays; ATP; Cell proliferation; Cytochrome c oxidase; Fibroblast; Light-emitting diode; Na⁺, K⁺-ATPase; Ouabain; Sodium azide;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Sciences (IECBES), 2010 IEEE EMBS Conference on

Conference_Location

Kuala Lumpur

Print_ISBN

978-1-4244-7599-5

Type

conf

DOI

10.1109/IECBES.2010.5742215

Filename

5742215