Analysis and experiment of centrifugal force for microfluidic ELISA CD platform

Author

Ibrahim, Fatimah ; Nozari, Ali A. ; Jahanshahi, Peyman ; Soi, Norhayati ; Rahman, Noorsaadah Abd ; Dawal, Siti Zawiah Md ; Kahar, Maria Kahar Bador Abdul ; Samra, Kameel Abi ; Madou, Marc

Author_Institution

Dept. of Biomed. Eng., Univ. of Malaya, Kuala Lumpur, Malaysia

fYear

2010

fDate

Nov. 30 2010-Dec. 2 2010

Firstpage

466

Lastpage

470

Abstract

This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD composed of 2 layers: the substrate layer is made of PMMA and the top layer is laminated with an Adhesive Sealing Films (ASF) or thermal seal. The PMMA substrate was fabricated by CNC micromachining. The ELISA CD consists of 7 reservoirs: waste, detection, serum, conjugate, washing solution, substrate and stopping solution. In the experiment, the reservoirs were filled with colour liquid and the fluid flow behaviour of the liquid in the CD was studied and monitored by a customized CD spin stand system equipped with visualization system. The experimental test results show an average of 9% error when compared with the theoretical calculation of burst frequency for all the reservoirs.

Keywords

bioMEMS; biochemistry; biomedical optical imaging; capillarity; enzymes; microchannel flow; micromachining; molecular biophysics; polymers; sealing materials; CNC micromachining; PMMA; adhesive sealing films; capillary force; centrifugal force; compact disc; conjugate reservoir; customized CD spin stand system; detection reservoir; enzyme-linked immunosorbent assay; fluid flow; microfluidic ELISA CD platform; serum reservoir; stopping solution reservoir; substrate reservoir; thermal seal; visualization system; washing solution reservoir; waste reservoir; Immune system; Immunity testing; Magnetic separation; Micromachining; Pumps; Reservoirs; Visualization; Burst frequency; ELISA; Lab on CD; Microfluidics; centrifugal force; microsystem;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Sciences (IECBES), 2010 IEEE EMBS Conference on

Conference_Location

Kuala Lumpur

Print_ISBN

978-1-4244-7599-5

Type

conf

DOI

10.1109/IECBES.2010.5742282

Filename

5742282