Initial Investigations with a Deep Ocean In Situ Sampler

A deep ocean sampler capable of aseptically sampling and maintaining a seawater sample under in situ conditions of pressure and temperature has been developed. The sampler comprises two interlocking subunits. A sequenced cam-driven triggering mechanism and a spring driven pump are integrated into one of the subunits. The second subunit consists of a 400 ml teflon-coated sample chamber that can be used for continuous incubation under elevated, i.e., in situ hydrostatic pressure. Nutrients may be added and mixed without decompression of the sample. The sampler has been tested in a high pressure chamber, on cruises in shallow depths, and in the Puerto Rican Trench.