Title :
Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds
Author :
Abdullah, Jaafar ; Ahmad, Musa ; Heng, Lee Yook ; Karuppiah, Nadarajah ; Sidek, Hamidah
Author_Institution :
Sch. of Chem. Sci. & Food Technol., Universiti Kebangsaan Malaysia, Selangor, Malaysia
Abstract :
Fabrication of biosensor for phenolic compounds determination employing optical method is described. The biosensor involved bi-layer which consist of immobilized 3-methyl-2-benzothiazolinone hydrazone (MBTH) in composite nafion/sol-gel silicate film and immobilized tyrosinase in chitosan film. The enzymatic oxidation product of phenolic compounds were stabilized through the formation of adduct with MBTH which can be monitored spectrophotometrically at wavelength of 490 run. The fabricated biosensor gave a linear response towards phenol, catechol and m-cresol in the concentration range of 5.0-74.0 μM, 5.0-91.0 μM, 9.0-120.0 μM with detection limit of 1.9, 2.1, 4.0 μM, respectively. The biosensor was stable for a duration of at least 3 months.
Keywords :
biosensors; chemical sensors; composite materials; enzymes; molecular biophysics; optical sensors; oxidation; polymer films; sol-gel processing; spectrochemical analysis; 490 nm; catechol; chitosan film; enzymatic oxidation; immobilized 3-methyl-2-benzothiazolinone hydrazone; immobilized MBTH; m-cresol; nafion/sol-gel silicate film; optical biosensor; phenol; phenolic compounds; spectrophotometry; tyrosinase; Biochemistry; Biomedical optical imaging; Biosensors; Food technology; Glass; Manufacturing processes; Monitoring; Optical device fabrication; Optical films; Oxidation;
Conference_Titel :
Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research, 2005 Asian Conference on
Print_ISBN :
0-7803-9370-8
DOI :
10.1109/ASENSE.2005.1564516
