Fabrication of an optical biosensor based on immobilized MBTH and tyrosinase for determination of phenolic compounds

Author

Abdullah, Jaafar ; Ahmad, Musa ; Heng, Lee Yook ; Karuppiah, Nadarajah ; Sidek, Hamidah

Author_Institution

Sch. of Chem. Sci. & Food Technol., Universiti Kebangsaan Malaysia, Selangor, Malaysia

fYear

2005

fDate

5-7 Sept. 2005

Firstpage

103

Lastpage

106

Abstract

Fabrication of biosensor for phenolic compounds determination employing optical method is described. The biosensor involved bi-layer which consist of immobilized 3-methyl-2-benzothiazolinone hydrazone (MBTH) in composite nafion/sol-gel silicate film and immobilized tyrosinase in chitosan film. The enzymatic oxidation product of phenolic compounds were stabilized through the formation of adduct with MBTH which can be monitored spectrophotometrically at wavelength of 490 run. The fabricated biosensor gave a linear response towards phenol, catechol and m-cresol in the concentration range of 5.0-74.0 μM, 5.0-91.0 μM, 9.0-120.0 μM with detection limit of 1.9, 2.1, 4.0 μM, respectively. The biosensor was stable for a duration of at least 3 months.

Keywords

biosensors; chemical sensors; composite materials; enzymes; molecular biophysics; optical sensors; oxidation; polymer films; sol-gel processing; spectrochemical analysis; 490 nm; catechol; chitosan film; enzymatic oxidation; immobilized 3-methyl-2-benzothiazolinone hydrazone; immobilized MBTH; m-cresol; nafion/sol-gel silicate film; optical biosensor; phenol; phenolic compounds; spectrophotometry; tyrosinase; Biochemistry; Biomedical optical imaging; Biosensors; Food technology; Glass; Manufacturing processes; Monitoring; Optical device fabrication; Optical films; Oxidation;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensors and the International Conference on new Techniques in Pharmaceutical and Biomedical Research, 2005 Asian Conference on

Print_ISBN

0-7803-9370-8

Type

conf

DOI

10.1109/ASENSE.2005.1564516

Filename

1564516