Title :
Potentiometric nano-grained TiO2: Ru-based nafion/uric acid biosensor
Author :
Chen, Cheng-Wei ; Chou, Jung-Chuan
Author_Institution :
Grad. Sch. of Eng. Sci. & Technol., Nat. Yunlin Univ. of Sci. & Technol., Yunlin, Taiwan
Abstract :
The potentiometric nafion/uric acid biosensor formed on nano-grained ruthenium-doped titanium dioxide (TiO2: Ru) sensing film are proposed in this investigation, which is employed to detect the uric acid concentration in the phosphate buffered saline (PBS) solution. SEM and XRD are used for observing surface morphology and crystalline phase of TiO2: Ru sensing film. The uricase (EC 1.7.3.3, 14.5 unit/mg) immobilized on 2 mm × 2 mm sensing area of the TiO2: Ru-based electrode by using the mixture of 3-glycidyloxypropyltrimethoxysilane (GPTS) and toluene with the volume ratio (GPTS : toluene = 1 : 4) is presented. The nafion/UA biosensor is measured in PBS uric acid solutions ranging between 2 mg/dl and 10 mg/dl whose sensitivity and linearity are 4.02 mV/(mg/dl) and 0.999, respectively.
Keywords :
X-ray diffraction; biosensors; ruthenium; scanning electron microscopy; surface morphology; titanium compounds; SEM; TiO2:Ru; XRD; crystalline phase; nano-grained ruthenium-doped titanium dioxide sensing film; phosphate buffered saline; potentiometric nafion/uric acid biosensor; potentiometric nano-grained biosensor; surface morphology; Biomembranes; Biosensors; Films; Linearity; Morphology; Surface morphology; 3-glycidy- loxypropyltrimethoxysilane (GPTS); nafion; ruthenium doped titanium dioxide (TiO2: Ru); uric acid (UA) biosensor; uricase;
Conference_Titel :
Nanoelectronics Conference (INEC), 2011 IEEE 4th International
Conference_Location :
Tao-Yuan
Print_ISBN :
978-1-4577-0379-9
Electronic_ISBN :
2159-3523
DOI :
10.1109/INEC.2011.5991744
