Potentiometric nano-grained TiO2: Ru-based nafion/uric acid biosensor

Author

Chen, Cheng-Wei ; Chou, Jung-Chuan

Author_Institution

Grad. Sch. of Eng. Sci. & Technol., Nat. Yunlin Univ. of Sci. & Technol., Yunlin, Taiwan

fYear

2011

fDate

21-24 June 2011

Firstpage

1

Lastpage

2

Abstract

The potentiometric nafion/uric acid biosensor formed on nano-grained ruthenium-doped titanium dioxide (TiO₂: Ru) sensing film are proposed in this investigation, which is employed to detect the uric acid concentration in the phosphate buffered saline (PBS) solution. SEM and XRD are used for observing surface morphology and crystalline phase of TiO₂: Ru sensing film. The uricase (EC 1.7.3.3, 14.5 unit/mg) immobilized on 2 mm × 2 mm sensing area of the TiO₂: Ru-based electrode by using the mixture of 3-glycidyloxypropyltrimethoxysilane (GPTS) and toluene with the volume ratio (GPTS : toluene = 1 : 4) is presented. The nafion/UA biosensor is measured in PBS uric acid solutions ranging between 2 mg/dl and 10 mg/dl whose sensitivity and linearity are 4.02 mV/(mg/dl) and 0.999, respectively.

Keywords

X-ray diffraction; biosensors; ruthenium; scanning electron microscopy; surface morphology; titanium compounds; SEM; TiO₂:Ru; XRD; crystalline phase; nano-grained ruthenium-doped titanium dioxide sensing film; phosphate buffered saline; potentiometric nafion/uric acid biosensor; potentiometric nano-grained biosensor; surface morphology; Biomembranes; Biosensors; Films; Linearity; Morphology; Surface morphology; 3-glycidy- loxypropyltrimethoxysilane (GPTS); nafion; ruthenium doped titanium dioxide (TiO2: Ru); uric acid (UA) biosensor; uricase;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanoelectronics Conference (INEC), 2011 IEEE 4th International

Conference_Location

Tao-Yuan

ISSN

2159-3523

Print_ISBN

978-1-4577-0379-9

Electronic_ISBN

2159-3523

Type

conf

DOI

10.1109/INEC.2011.5991744

Filename

5991744