Fast-scanning two-photon fluorescence imaging using a microelectromechanical systems (MEMS) two-dimensional scanning mirror

Author

Piyawattanametha, Wibool ; Barretto, Robert P J ; Ko, Tony H. ; Flusberg, Benjamin A. ; Cocker, Eric D. ; Ra, Hyejun ; Lee, Daesung ; Solgaard, Olav ; Schnitzer, Mark J.

Author_Institution

James H. Clark Center for Biomed. Eng.&Sci., Stanford Univ., Stanford, CA

fYear

2006

fDate

21-26 May 2006

Firstpage

1

Lastpage

2

Abstract

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce laser-scanning instrumentation based on a microelectromechanical systems (MEMS) scanner and describe two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.5 kHz.

Keywords

endoscopes; laser mirrors; micromechanical devices; optical scanners; photon-photon interactions; 2D scanning mirror; MEMS; fast-scanning two-photon fluorescence imaging; laser-scanning instrumentation; microelectromechanical systems; microendoscopy; photon-photon microscopy; size limitations; Biomedical engineering; Biomedical optical imaging; Endoscopes; Fluorescence; Microelectromechanical systems; Micromechanical devices; Mirrors; Optical imaging; Optical microscopy; Optical scattering;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2006 and 2006 Quantum Electronics and Laser Science Conference. CLEO/QELS 2006. Conference on

Conference_Location

Long Beach, CA

Print_ISBN

978-1-55752-813-1

Electronic_ISBN

978-1-55752-813-1

Type

conf

DOI

10.1109/CLEO.2006.4627825

Filename

4627825