Fingerprint Chromatogram and Fuzzy Calculation for Quality Control of Shenrong Tonic Wine

With computer software and fuzzy calculation, we determined several key compounds and established fingerprint to control the stability of food products. High performance liquid chromatography (HPLC) was used to establish the fingerprint chromatogram of Shenrong tonic wine to control its quality and stability, and to detect possible counterfeits. We used reverse phase C-18 column, equivalent elution and detection wavelength at 259 nm. The chromatographic fingerprint was established by using sample chromatography of 10 different production batches to calculate the relative retention time ¿ and the relative area Ar of each peak respectively, and 11 peaks of common characteristic had been found. Cosine method was used to calculate the similarity by which the comparative study was done on various batches. The results showed that the method was convenient and applicable for the quality evaluation of Chinese herb tonic wine.