Title :
A novel magnetic tweezers for manipulation of a single DNA molecule
Author :
Chiou, Chi-Han ; Tseng, Zhao-Fu ; Lee, Gwo-Bin
Author_Institution :
Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan
Abstract :
We report a novel magnetic tweezers for manipulation of a single DNA molecule. The micromachined DNA manipulator can stretch and rotate a single DNA molecule using arrayed microcoils. Key platform technologies including localized DNA immobilization, microcoil fabrication and microfluidics, have been integrated to form the magnetic DNA tweezers. A single DNA molecule is specifically attached onto a magnetic bead and a gold surface and manipulated under a magnetic field generated by built-in hexagonally-aligned microcoils. A highly effective method for the construction of DNA two sticky ends is developed, which is compatible with MEMS technologies. We have successfully demonstrated the rotation of the tethered-bead DNA molecule linked to the gold pattern by circular permutation of the currents applied to the microcoils.
Keywords :
DNA; biological techniques; biomolecular electronics; microfluidics; micromachining; micromanipulators; molecular biophysics; Au; DNA immobilization; MEMS technologies; deoxyribonucleic acid; gold surface; magnetic DNA tweezers; magnetic bead; magnetic field; microcoil fabrication; microfluidics; micromachined DNA manipulator; micromechanical devices; single DNA molecule; Atom optics; Atomic force microscopy; Biomedical optical imaging; Coils; Copper; DNA; Fabrication; Force measurement; Gold; Microfluidics;
Conference_Titel :
Micro Electro Mechanical Systems, 2004. 17th IEEE International Conference on. (MEMS)
Print_ISBN :
0-7803-8265-X
DOI :
10.1109/MEMS.2004.1290659
