Title :
Penetration depth in multiphoton fluorescence microscopy
Author :
Gu, Min ; Gan, Xiaosong
Author_Institution :
Centre for Micro-Photonics, Swinburne Univ. of Technol., Hawthorn, Vic., Australia
Abstract :
In this paper, we demonstrate that the penetration depth under two-photon excitation is limited by the strength of two-photon fluorescence and is not necessarily larger than that under single-photon excitation, although image resolution is much higher in the former case.
Keywords :
Mie scattering; fluorescence; image resolution; optical microscopy; two-photon processes; image resolution; multiphoton fluorescence microscopy; penetration depth; two-photon excitation; Fluorescence; Image resolution; Laser excitation; Light scattering; Microscopy; Mie scattering; Optical scattering; Particle scattering; Rayleigh scattering; Scattering parameters;
Conference_Titel :
Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on
Print_ISBN :
0-7803-8676-0
DOI :
10.1109/APBP.2004.1412284
