Title :
An investigation of thermally induced collagen structural transitions by second harmonic generation microscopy
Author :
Sun, Yen ; Sun-Jan Lin ; Hsiao, Chih-Yuan ; Lo, Wen ; Jee, Shiou-Hwa ; Dong, Chen-Yuan
Author_Institution :
Dept. of Phys., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
In this work, the thermal disruption of type I collagen is investigated using second harmonic generation (SHG) microscopy. We investigated the effects of second harmonic generation images and intensity of rat tail tendon in the temperature range from 25 to 60°C. We found a structural transition at 57°C where a drop in second harmonic intensity is correlated to a disorganization of the collagen fibers. Our results suggest that SHG microscopy is an effective technique to monitor thermally induced collagen structural changes for biomedical applications.
Keywords :
biomedical optical imaging; biothermics; macromolecules; molecular biophysics; molecular configurations; multiphoton spectroscopy; optical harmonic generation; optical microscopy; proteins; 25 to 60 C; SHG microscopy; biomedical applications; collagen fiber disorganization; rat tail tendon; second harmonic generation microscopy; thermal disruption; thermally induced collagen structural transitions; type I collagen; Biomedical imaging; Fluorescence; Frequency conversion; Laser stability; Optical harmonic generation; Optical imaging; Optical microscopy; Tail; Temperature distribution; Tendons;
Conference_Titel :
Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on
Print_ISBN :
0-7803-8676-0
DOI :
10.1109/APBP.2004.1412339
