Title :
A micromanipulation platform based on magnetic tweezers and total internal reflection fluorescence microscopy for cell mechanical studies
Author :
Lee, Chaug-Chun ; Chien, Cheng-Hao ; Wen, Liang-Kung ; Dong, Chen-Yuan
Author_Institution :
Dept. of Phys., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
We developed a manipulation platform, which combines magnetic tweezers and the incubating system, with total internal reflection fluorescence microscopy (TIRFM). Our system can generate forces up to the nN range and such forces can be used to observe and characterize cell mechanical responses to external forces. Combined with TIRFM, cell surface stress-strain phenomena can also be investigated.
Keywords :
biomechanics; cell motility; fluorescence; micromanipulators; optical microscopy; cell mechanical study; cell surface stress-strain phenomena; fluorescence microscopy; magnetic tweezers; micromanipulation platform; total internal reflection; Biomembranes; Biophysics; Cells (biology); Fluorescence; Force control; Instruments; Laboratories; Magnetic force microscopy; Physics; Reflection;
Conference_Titel :
Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on
Print_ISBN :
0-7803-8676-0
DOI :
10.1109/APBP.2004.1412350
