Title :
Image driven cell manipulation using optical dielectrophoresis (ODEP)
Author :
Yen-Sheng Lu ; Huang, Yuan-Peng ; Yeh, J. Andrew ; Lee, Chengkuo
Author_Institution :
Inst. of MicroElectroMech. Syst., Nat. Tsing Hua Univ., Hsinchu, Taiwan
Abstract :
We demonstrated a novel approach to trigger the dielectrophoretic force to manipulate biological cells via projected flash images. The attractive and repulsive force regions are formed due to effectively initiated dielectrophoretic force, where these regions are the bright and dark area among a projected image on a liquid reservoir depending on the polarization states. In present study, the attractive force draw in live cells, saccharomyces cerevisiae, and live cells can be manipulated inside reservoir without any microchannel confinement in a programmable manner. The relation between distance from the boundary and average velocity is also discussed.
Keywords :
biomedical optical imaging; cellular biophysics; electrophoresis; light polarisation; biological cell; flash image; image driven cell manipulation; liquid reservoir; microchannel confinement; optical dielectrophoresis; polarization state; saccharomyces cerevisiae; Asia; Biomedical optical imaging; Dielectrophoresis; Glass; Laser beams; Microelectromechanical systems; Optical control; Polarization; Reservoirs; Silicon;
Conference_Titel :
Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on
Print_ISBN :
0-7803-8676-0
DOI :
10.1109/APBP.2004.1412370