Title :
Ultrafast excited-state dynamics of genetic fusions: The Green Fluorescent Protein as a folding reporter
Author :
Didier, P. ; Guidoni, L. ; Weiss, E.
Author_Institution :
Lab. IPCMS, CNRS-ULP-ECPM, Strasbourg
Abstract :
We investigated the excited-state ultra-fast dynamics of genetic fusions that link a Green Fluorescent Protein to an antibody fragment. Spectrally resolved pump-probe spectroscopy allowed us to discriminate between biolocally-active soluble and inactive insoluble antibody fusions.
Keywords :
excited states; fluorescence; genetics; high-speed optical techniques; molecular biophysics; proteins; solubility; antibody fragment; antibody fusions; folding reporter; genetic fusions; green fluorescent protein; solubility; spectrally resolved pump-probe spectroscopy; ultrafast excited-state dynamics; Absorption; Biomedical optical imaging; Fluorescence; Genetics; Laser excitation; Optical pumping; Proteins; Spectroscopy; Stimulated emission; Ultrafast optics; (320.7130) Ultrafast processes in condensed matter; (320.7150) Ultrafast spectroscopy;
Conference_Titel :
Lasers and Electro-Optics, 2006 and 2006 Quantum Electronics and Laser Science Conference. CLEO/QELS 2006. Conference on
Conference_Location :
Long Beach, CA
Print_ISBN :
978-1-55752-813-1
Electronic_ISBN :
978-1-55752-813-1
DOI :
10.1109/CLEO.2006.4628949
