Handling of single DNA molecule using electric field and laser beam

Author

Mizuno, Akira ; Nishioka, Masateru ; Tanizoe, Tomoki ; Katsura, Shinji

Author_Institution

Dept. of Ecological Eng., Toyohashi Univ. of Technol., Japan

fYear

1994

fDate

2-6 Oct 1994

Firstpage

1477

Abstract

Accurate handling of a single DNA molecule will support a novel technique for sequencing of a long DNA molecule. The opto-electrostatic micromanipulation (OEMM) method has been applied to handle single DNA molecules. The DNA molecule is frequently changing its position and shape due to Brownian motion, therefore precise handling is difficult. Gelation of the sample by agarose gel is effective to suppress Brownian motion. Fundamental handling techniques for the single DNA molecule in agarose gel were demonstrated: (1) fixation with stretched shape; (2) cutting by ultraviolet laser, (3) isolation and recovery into the capillary (i.d. 15 μm). Amplification of the recovered DNA fragment by the PCR (polymerase chain reaction) method is the next step of this study. The micromanipulation may contribute to the speeding up of the DNA sequencing

Keywords

DNA; biological techniques; electric fields; laser beam applications; molecular biophysics; 15 mum; Brownian motion suppression; DNA sequencing speeding up; accurate handling; agarose gel; electric field technique; laser beam technique; optoelectrostatic micromanipulation method; polymerase chain reaction method; sample gelation; single DNA molecule handling; stretched shape; ultraviolet laser cutting; Biomedical optical imaging; Biotechnology; DNA; Dielectric measurements; Dielectrophoresis; Electrostatics; Laser beams; Optical feedback; Shape; Temperature measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Industry Applications Society Annual Meeting, 1994., Conference Record of the 1994 IEEE

Conference_Location

Denver, CO

Print_ISBN

0-7803-1993-1

Type

conf

DOI

10.1109/IAS.1994.377620

Filename

377620