An MCP-based system for beta autoradiography

Author

Lees, J.E. ; Pearson, J.F. ; Fraser, G.W. ; Hales, J.M. ; Richards, P.G.

Author_Institution

Dept. of Phys. & Astron., Leicester Univ., UK

Volume

3

fYear

1998

fDate

1998

Firstpage

1513

Abstract

Describes a pre-commercial prototype autoradiography system which can be used to image a variety of radiolabelled biological samples, ranging from lymphocyte proliferation assays to semi-thin whole body tissue slices. These samples can be imaged with high spatial resolution (~70 microns) and high sensitivity (e.g. 3H-minimum detectable activity 0.001 Bq in 20 hours) over a wide (5-6 orders of magnitude) dynamic range. Specimens are automatically presented, through a vacuum load-lock, to an imaging microchannel plate (MCP) detector operating in high (~10^-6 mbar) vacuum. The authors present images obtained from tritium labelled samples and discuss the importance of the new system for a variety of biological techniques

Keywords

biological techniques; biological tissues; blood; radioisotope imaging; 0.001 becquerel; 20 h; 70 mum; MCP-based system; automatically presented specimens; beta autoradiography; dynamic range; high vacuum; imaging microchannel plate detector; lymphocyte proliferation assays; minimum detectable activity; pre-commercial prototype autoradiography system; radiolabelled biological samples; semi-thin whole body tissue slices; spatial resolution; tritium labelled samples; vacuum load-lock; Cameras; Detectors; Dynamic range; Electrokinetics; High-resolution imaging; Microchannel; Pixel; Pollution measurement; Prototypes; Spatial resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

Nuclear Science Symposium, 1998. Conference Record. 1998 IEEE

Conference_Location

Toronto, Ont.

ISSN

1082-3654

Print_ISBN

0-7803-5021-9

Type

conf

DOI

10.1109/NSSMIC.1998.773831

Filename

773831