Detection of Nonylphenol by Exonuclease Protection Fluorescent Quantitative PCR

It is established that a fast, sensitive exonuclease protection fluorescent quantitative PCR assay for detection of nonyl phenol. Different concentrations of nonylphenol can combine with estrogen receptors extracted from goldfish liver and synthetic DNA with specific primer to format the complexes which are used as templates for fluorescence quantitative PCR. Using linear regression analysis with quantitative DNA standard curve and Ct and the curve of nonylphenol concentration, it is found that the relationship between nonylphenol concentration and the starting copy number of DNA concentration which can be used to detect nonylphenol. This method can achieve the minimum detection limit of 3.33ng/L with high sensitivity, it can be used as a rapid detection method for nonylphenol.