Simultaneous Determination of Ten Kinds of Polybrominated Diphenyl Ethers (PBDEs) in Human Serum by Gas Chromatography: Mass Spectrometry

The aim of this paper was to establish a method to determine ten kinds of PBDEs in human serum simultaneously. The linear range of measurement had good relationship (r>;0.992). Blood samples were centrifuged and extracted. Hexane was respectively eluted through HLB column after serum was changed by urea and then purified with anhydrous sodium sulfate(Na₂SO₄)for analysis. Gas chromatography-mass spectrometry selected ion monitoring (GC-MSSIM)detection and quantitative analysis based on external standard were used to detect ten kinds of PBDEs in human serum simultaneously. The results indicated that the average recovery of the external standard was from 75.5% to 110%. The detection limit of the method was in the range of 0.001-C 0.05 ng, The detection method of ten PBDE scongeners in human serum was sensitive with high reproducible of satisfied recoveries.