Title :
Anomalous diffusion of kv2.1 channels observed by single molecule tracking in live cells
Author :
Weigel, Aubrey V. ; Tamkun, Michael M. ; Krapf, Diego
Author_Institution :
Sch. of Biomed. Eng., Colorado State Univ., Fort Collins, CO, USA
fDate :
Aug. 31 2010-Sept. 4 2010
Abstract :
Kv2.1 are voltage gated potassium channels that form long-lived clusters on the surface of mammalian cells. We have used single molecule tracking to study the interesting dynamics of these channels in live HEK cells. Both the channels inside the clusters and non-clustering channels are found to follow anomalous subdiffusion. The effect of actin cytoskeleton on the diffusion properties of the channels is also investigated in the presence of cytochalasin D, a F-actin binding drug that blocks actin polymerization.
Keywords :
biochemistry; biodiffusion; bioelectric phenomena; biomembrane transport; drugs; kidney; polymerisation; proteins; F-actin binding drug; Kv2.1 channels; actin cytoskeleton; actin polymerization; anomalous diffusion; cytochalasin D; live HEK cells; long-lived clusters; mammalian cells; single molecule tracking; voltage gated potassium channels; Biomembranes; Cells (biology); Drugs; Fluorescence; Imaging; Quantum dots; Trajectory; Actins; Algorithms; Cell Line; Cluster Analysis; Cytochalasin D; Diffusion; Green Fluorescent Proteins; Humans; Microscopy, Fluorescence; Quantum Dots; Reproducibility of Results; Shab Potassium Channels; Time Factors; Transfection;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE
Conference_Location :
Buenos Aires
Print_ISBN :
978-1-4244-4123-5
DOI :
10.1109/IEMBS.2010.5626150
