Diagnosis of foot-and-mouth disease by electrochemical enzyme-linked immunoassay

Author

Longinotti, Gloria ; Ybarra, Gabriel ; Lloret, Paulina ; Moina, Carlos ; Ciochinni, Andrés ; Serantes, Diego Rey ; Malatto, Laura ; Roberti, Mariano ; Tropea, Salvador ; Fraigi, Liliana

Author_Institution

Inst. Nac. de Tecnol. Ind., Argentina

fYear

2010

fDate

Aug. 31 2010-Sept. 4 2010

Firstpage

674

Lastpage

676

Abstract

The development of an inmunosensor for the point-of-care detection of the foot-and-mouth cattle disease is presented. The detector is based on an ELISA method with electrochemical detection. A non-structural protein, 3ABC, is used to selectively detect antibodies is used to selectively detect anti-3ABC antibodies produced after infection. The biological test is performed onto a screen printed electrodes. A dedicated small, portable potentiostat is employed for the control of the sensors, as well as data acquisition, processing, and storage.

Keywords

biochemistry; biosensors; data acquisition; diseases; electrochemical sensors; enzymes; patient diagnosis; 3ABC; ELISA method; antibodies; data acquisition; data processing; data storage; electrochemical detection; electrochemical enzyme-linked immunoassay; foot-and-mouth disease; inmunosensor; nonstructural protein; patient diagnosis; point-of-care detection; portable potentiostat; screen printed electrodes; Biosensors; Cows; Diseases; Electrodes; Gold; Immune system; Proteins; Animals; Antibodies; Conductometry; Enzyme-Linked Immunosorbent Assay; Equipment Design; Equipment Failure Analysis; Foot-and-Mouth Disease; Humans; Point-of-Care Systems; Viral Nonstructural Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE

Conference_Location

Buenos Aires

ISSN

1557-170X

Print_ISBN

978-1-4244-4123-5

Type

conf

DOI

10.1109/IEMBS.2010.5626230

Filename

5626230