Identification of intracellular calcium dynamics in stimulated cardiomyocytes

Author

Vallmitjana, A. ; Barriga, M. ; Nenadic, Z. ; Llach, A. ; Alvarez-Lacalle, E. ; Hove-Madsen, L. ; Benitez, R.

Author_Institution

Autom. Control Dept., Univ. Politec. de Catalunya (UPC), Barcelona, Spain

fYear

2010

fDate

Aug. 31 2010-Sept. 4 2010

Firstpage

68

Lastpage

71

Abstract

We have developed an automatic method for the analysis and identification of dynamical regimes in intracellular calcium patterns from confocal calcium images. The method allows the identification of different dynamical patterns such as spatially concordant and discordant alternans, irregular behavior or phase-locking regimes such as period doubling or halving. The method can be applied to the analysis of different cardiac pathologies related to anomalies at the cellular level such as ventricular reentrant arrhythmias.

Keywords

biomedical optical imaging; calcium; cardiology; cellular biophysics; diseases; feature extraction; fluorescence; medical image processing; muscle; physiology; cardiac pathology; confocal calcium images; discordant alternans; dynamical patterns; feature extraction; fluorescence; intracellular calcium dynamics; phase-locking; spatially concordant alternans; stimulated cardiomyocytes; ventricular reentrant arrhythmias; Calcium; Cardiology; Feature extraction; Heart; Pixel; Principal component analysis; Transient analysis; Calcium; Cells, Cultured; Histocytochemistry; Humans; Image Processing, Computer-Assisted; Intracellular Space; Microscopy, Confocal; Myocytes, Cardiac; Principal Component Analysis;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE

Conference_Location

Buenos Aires

ISSN

1557-170X

Print_ISBN

978-1-4244-4123-5

Type

conf

DOI

10.1109/IEMBS.2010.5626238

Filename

5626238