Single-molecule imaging in solution with total internal reflection excitation

Author

Parker, W.C. ; Davis, Lloyd M. ; Williams, J.G.K. ; Middendorf, L.R. ; Bashford, C.R.

Author_Institution

Center for Laser Applications, Tennessee Univ. Space Inst., Tullahoma, TN, USA

fYear

2000

fDate

7-12 May 2000

Firstpage

502

Lastpage

503

Abstract

Summary form only given.The detection of individual single-chromophore molecules in aqueous solution, first demonstrated with picoliter-sized sample volumes, and soon after with femtoliter-sized volumes, has become routinely achievable. More recently, the imaging of individual single-chromophore molecules in very thin layers of aqueous solution, using epifluorescence and total internal reflection (TIR) microscopies has been reported.

Keywords

CCD image sensors; biomedical imaging; molecular biophysics; optical microscopy; reflectivity; aqueous solution; epifluorescence; femtoliter-sized volumes; individual single-chromophore molecule detection; individual single-chromophore molecules; picoliter-sized sample volumes; single-molecule imaging; total internal reflection excitation; total internal reflection microscopy; very thin layers; Bones; Fiber lasers; Laser ablation; Laser excitation; Laser surgery; Optical control; Optical reflection; Optical scattering; Proteins; Surges;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2000. (CLEO 2000). Conference on

Conference_Location

San Francisco, CA, USA

Print_ISBN

1-55752-634-6

Type

conf

DOI

10.1109/CLEO.2000.907311

Filename

907311