Title :
Precise detection of singly mismatched DNA with functionalized diamond electrolyte solution gate FET.
Author :
Kuga, Shoma ; Tajima, Shinya ; Yang, Jung-hoon ; Hirama, Kazuyuki ; Kawarada, Hiroshi
Author_Institution :
Sch. of Sci. & Engr., Waseda Univ., Tokyo
Abstract :
The DC operation of diamond electrolyte solution gate FETs (SGFETs) and discrimination of complementary and singly mismatched DNAs in solution were demonstrated. The transconductance (gm), which is the sensitivity for detecting the hybridization of DNA on SGFET, was increased from 60 muS/mm to 9.5 mS/mm by miniaturization of FET. Hybridization of target DNA with probe DNA was detected by the gate potential shift caused by the negative charges on DNA immobilized on the channel surface. The change in gate potential caused by the hybridization of complementary DNA was about 25 mV. The ratio of change in gate potential by hybridization of complementary and single-mismatched DNA was 3:1 on diamond SGFET.
Keywords :
DNA; biomolecular electronics; electrolytes; field effect transistors; C; DNA; diamond electrolyte solution gate; gate potential; solution gate FET; transconductance; Biosensors; Capacitance; Conductivity; DNA; FETs; Insulation; Intrusion detection; MOSFET circuits; Probes; Transconductance;
Conference_Titel :
Electron Devices Meeting, 2008. IEDM 2008. IEEE International
Conference_Location :
San Francisco, CA
Print_ISBN :
978-1-4244-2377-4
Electronic_ISBN :
8164-2284
DOI :
10.1109/IEDM.2008.4796731
