Precise detection of singly mismatched DNA with functionalized diamond electrolyte solution gate FET.

Author

Kuga, Shoma ; Tajima, Shinya ; Yang, Jung-hoon ; Hirama, Kazuyuki ; Kawarada, Hiroshi

Author_Institution

Sch. of Sci. & Engr., Waseda Univ., Tokyo

fYear

2008

fDate

15-17 Dec. 2008

Firstpage

1

Lastpage

4

Abstract

The DC operation of diamond electrolyte solution gate FETs (SGFETs) and discrimination of complementary and singly mismatched DNAs in solution were demonstrated. The transconductance (gm), which is the sensitivity for detecting the hybridization of DNA on SGFET, was increased from 60 muS/mm to 9.5 mS/mm by miniaturization of FET. Hybridization of target DNA with probe DNA was detected by the gate potential shift caused by the negative charges on DNA immobilized on the channel surface. The change in gate potential caused by the hybridization of complementary DNA was about 25 mV. The ratio of change in gate potential by hybridization of complementary and single-mismatched DNA was 3:1 on diamond SGFET.

Keywords

DNA; biomolecular electronics; electrolytes; field effect transistors; C; DNA; diamond electrolyte solution gate; gate potential; solution gate FET; transconductance; Biosensors; Capacitance; Conductivity; DNA; FETs; Insulation; Intrusion detection; MOSFET circuits; Probes; Transconductance;

fLanguage

English

Publisher

ieee

Conference_Titel

Electron Devices Meeting, 2008. IEDM 2008. IEEE International

Conference_Location

San Francisco, CA

ISSN

8164-2284

Print_ISBN

978-1-4244-2377-4

Electronic_ISBN

8164-2284

Type

conf

DOI

10.1109/IEDM.2008.4796731

Filename

4796731