Title :
Preliminary investigations on laminin coatings for flexible polyimide/platinum thin films for PNS applications
Author :
Bossi, S. ; Benvenuto, A. ; Wieringa, P. ; Di Pino, G. ; Guglielmelli, E. ; Boretius, T. ; Stieglitz, T. ; Navarro, X. ; Micera, S.
Author_Institution :
Adv. Robot. Technol. & Syst. Lab., Scuola Superiore Sant´´Anna, Pontedera, Italy
fDate :
Aug. 31 2010-Sept. 4 2010
Abstract :
The aim of this work was to investigate the possibility to obtain stable bioactive coatings for polyimide/platinum neural interfaces based on thin film technology for applications into the peripheral nervous system (PNS). Laminin (LI), a glycoprotein of the extracellular matrix, which guides and promotes differentiation and growth of neurons, was selected to deposit bioactive coatings. Dip-coating was performed on dummy structures at different LI concentrations. Indirect methods allowed to identify and characterize laminin on coated samples. Mechanical stability was also confirmed by indirect evaluations. Pilot experiments with differentiated PC12 cells, by the addition of nerve growth factor (NGF), showed improved neurite outgrowth on the coated probes compared to bare polyimide samples.
Keywords :
biomedical materials; cellular biophysics; dip coating; mechanical stability; metallic thin films; molecular biophysics; neurophysiology; platinum; polymer films; proteins; bioactive coatings; differentiated PC12 cells; dip-coating; dummy structures; extracellular matrix; flexible polyimide-platinum thin films; glycoprotein; laminin coatings; mechanical stability; nerve growth factor; neuron differentiation; peripheral nervous system; Coatings; Electrodes; Microscopy; Platinum; Polyimides; Surface treatment; Animals; Cell Survival; Coated Materials, Biocompatible; Elastic Modulus; Electrodes, Implanted; Equipment Design; Equipment Failure Analysis; Humans; Laminin; Materials Testing; Membranes, Artificial; PC12 Cells; Pattern Recognition, Automated; Peripheral Nerves; Platinum; Rats; Resins, Synthetic;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE
Conference_Location :
Buenos Aires
Print_ISBN :
978-1-4244-4123-5
DOI :
10.1109/IEMBS.2010.5626831
