Preliminary investigations on laminin coatings for flexible polyimide/platinum thin films for PNS applications

Author

Bossi, S. ; Benvenuto, A. ; Wieringa, P. ; Di Pino, G. ; Guglielmelli, E. ; Boretius, T. ; Stieglitz, T. ; Navarro, X. ; Micera, S.

Author_Institution

Adv. Robot. Technol. & Syst. Lab., Scuola Superiore Sant´´Anna, Pontedera, Italy

fYear

2010

fDate

Aug. 31 2010-Sept. 4 2010

Firstpage

1527

Lastpage

1530

Abstract

The aim of this work was to investigate the possibility to obtain stable bioactive coatings for polyimide/platinum neural interfaces based on thin film technology for applications into the peripheral nervous system (PNS). Laminin (LI), a glycoprotein of the extracellular matrix, which guides and promotes differentiation and growth of neurons, was selected to deposit bioactive coatings. Dip-coating was performed on dummy structures at different LI concentrations. Indirect methods allowed to identify and characterize laminin on coated samples. Mechanical stability was also confirmed by indirect evaluations. Pilot experiments with differentiated PC12 cells, by the addition of nerve growth factor (NGF), showed improved neurite outgrowth on the coated probes compared to bare polyimide samples.

Keywords

biomedical materials; cellular biophysics; dip coating; mechanical stability; metallic thin films; molecular biophysics; neurophysiology; platinum; polymer films; proteins; bioactive coatings; differentiated PC12 cells; dip-coating; dummy structures; extracellular matrix; flexible polyimide-platinum thin films; glycoprotein; laminin coatings; mechanical stability; nerve growth factor; neuron differentiation; peripheral nervous system; Coatings; Electrodes; Microscopy; Platinum; Polyimides; Surface treatment; Animals; Cell Survival; Coated Materials, Biocompatible; Elastic Modulus; Electrodes, Implanted; Equipment Design; Equipment Failure Analysis; Humans; Laminin; Materials Testing; Membranes, Artificial; PC12 Cells; Pattern Recognition, Automated; Peripheral Nerves; Platinum; Rats; Resins, Synthetic;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE

Conference_Location

Buenos Aires

ISSN

1557-170X

Print_ISBN

978-1-4244-4123-5

Type

conf

DOI

10.1109/IEMBS.2010.5626831

Filename

5626831