Title :
Third-harmonic microscopy in biology
Author :
Yelin, D. ; Silberberg, Y.
Author_Institution :
Dept. of Phys. of Complex Syst., Weizmann Inst. of Sci., Rehovot, Israel
Abstract :
Summary form only given. A laser scanning microscope using third-harmonic generation (THG) is shown to produce high-resolution images of transparent biological tissues. In THG microscopy, third-harmonic light is generated at the focal point of a tightly focused short-pulse laser beam. The image is generated by scanning the sample point-by-point. Due to its nonlinear nature, third harmonic light is generated only at the vicinity of the focal point, which allows optical sectioning of the sample with high lateral resolution. Since all materials have non-vanishing third-order susceptibilities, this method can he utilized as a general-purpose microscopy technique.
Keywords :
biological techniques; biological tissues; image resolution; nonlinear optical susceptibility; optical focusing; optical harmonic generation; optical microscopy; THG; THG microscopy; biology; focal point; general-purpose microscopy technique; high lateral resolution; high-resolution images; laser scanning microscope; optical sectioning; third-harmonic generation; third-harmonic light; third-harmonic microscopy; third-order susceptibilities; tightly focused short-pulse laser beam; transparent biological tissues; Biomedical optical imaging; Fluorescence; Impurities; Laser excitation; Light sources; Optical detectors; Optical microscopy; Optical sensors; Probes; Ring lasers;
Conference_Titel :
Quantum Electronics Conference, 2000. Conference Digest. 2000 International
Conference_Location :
Nice, France
Print_ISBN :
0-7803-6318-3
DOI :
10.1109/IQEC.2000.908163
