Monolithically integrated biosensors based on Frequency-Resolved Mach-Zehnder Interferometers for multi-analyte determinations

Author

Petrou, Panagiota S. ; Kitsara, Maria ; Makarona, Eleni ; Raptis, Ioannis ; Kakabakos, Sotirios E. ; Stoffer, Remco ; Jobst, Gerhard ; Misiakos, Konstantinos

Author_Institution

Immunoassay/Immunosensors Lab., Inst. of Radioisotopes & Radiodiagnostic Products, Aghia Paraskevi, Greece

fYear

2010

fDate

Aug. 31 2010-Sept. 4 2010

Firstpage

298

Lastpage

301

Abstract

The application of fully monolithically-integrated Mach-Zehnder interferometer arrays fabricated by standard silicon technology to the label-free detection of analytes is introduced. Detection with the presented biosensor is based on a novel concept, the Frequency-Resolved Mach-Zehnder Interferometry (FR-MZI). In addition, a smart encapsulation based on an appropriately designed microfluidic system and performed at the wafer scale scheme for the easy delivery of the samples to be analyzed is demonstrated. Testing of the FR-MZI biosensors with model binding assays demonstrated the detection of streptavidin binding to immobilized biotin at concentrations in the sub nM range. This is the first experimental demonstration of the FR-MZI concept as well as the first demonstration of a monolithically fully-integrated MZI biosensor.

Keywords

Mach-Zehnder interferometers; bioMEMS; biosensors; chemical sensors; microfluidics; microsensors; molecular biophysics; monolithic integrated circuits; proteins; FR-MZI; frequency resolved Mach-Zehnder interferometers; immobilized biotin; label free analyte detection; microfluidic system; model binding assays; monolithically integrated biosensors; multianalyte determinations; smart encapsulation; standard silicon technology; streptavidin binding detection; Biomedical optical imaging; Biosensors; Light emitting diodes; Optical interferometry; Optical sensors; Silicon; Biosensing Techniques; Biotin; Equipment Design; Interferometry; Microfluidic Analytical Techniques; Signal Processing, Computer-Assisted; Streptavidin;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE

Conference_Location

Buenos Aires

ISSN

1557-170X

Print_ISBN

978-1-4244-4123-5

Type

conf

DOI

10.1109/IEMBS.2010.5627478

Filename

5627478