Title :
Effect of sustained release of bone morphogenetic protein on osteogenic expression of mesenchymal stem cells
Author :
Jabbari, Esmaiel
Author_Institution :
Univ. of South Carolina, USA
fDate :
Aug. 31 2010-Sept. 4 2010
Abstract :
The objective of this work was to investigate the effect of sustained release of rhBMP-2, grafted to biodegradable nanoparticles (NPs) based on osteogenic differentiation of bone mesenchymal stem (BMS) cells. The release kinetics of rhBM-2 was linear with incubation time in the first two weeks. rhBMP-2 grafted NPs were as effective in inducing mineralization as the rhBMP-2 directly added to the culture media. rhBMP-2 grafted NPs had much higher expression of osteogenic markers osteopontin (OP) and osteocalcin (OC), compared to the protein directly added to culture media.
Keywords :
biodegradable materials; biomedical materials; bone; cellular biophysics; genetics; molecular biophysics; nanobiotechnology; nanoparticles; proteins; biodegradable nanoparticles; bone morphogenetic protein sustained release; incubation time; mesenchymal stem cells; mineralization; osteocalcin; osteogenic expression; osteogenic markers osteopontin; recombinant human bone morphogenetic protein-2; rhBMP-2 grafted NP; time 2 wk; Bones; Calcium; Degradation; Media; Mineralization; Proteins; Suspensions; Animals; Bone Morphogenetic Protein 2; Cell Differentiation; Cells, Cultured; Delayed-Action Preparations; Male; Mesenchymal Stem Cells; Osteoblasts; Osteogenesis; Rats; Rats, Wistar;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2010 Annual International Conference of the IEEE
Conference_Location :
Buenos Aires
Print_ISBN :
978-1-4244-4123-5
DOI :
10.1109/IEMBS.2010.5627505
